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sigB [2019-02-19 19:22:11]
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sigB [2019-02-19 19:22:11]

[SW|RNA polymerase] [SW|sigma factor] SigB
locus
BSU04730
pI
5.42
mw
29.99 kDa
protein length
262 aa Sequence Blast
gene length
789 bp Sequence Blast
function
general stress response
product
[SW|RNA polymerase] [SW|sigma factor] SigB
essential
no
synonyms
rpoF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

    • Gene

    Coordinates
    522,862 523,650

    The protein

    Protein family

  • SigB subfamily (according to Swiss-Prot)

    • Structure

  • [PDB|1L0O] ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]-[protein|search|SpoIIAB ]complex from Geobacillus stearothermophilus, 32% identity) [pubmed|11955433]

    • Expression and Regulation

    Operons

    genes
    [gene|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR]-[gene|841CE7FCA4E84445830CA18F9856F6F30014E3BB|rsbS]-[gene|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT]-[gene|0B8381D2C724DAA724E4AFC67000F12AD112D13D|rsbU]-[gene|AC64DA463250A090A62E50901EFE653C8F963872|rsbV]-[gene|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|rsbW]-[gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]
    description
    [Pubmed|8002610]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8002610], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|20454630], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • ''[protein|search|rsbV]:'' induced by stress ([protein|search|SigB]) [Pubmed|11544224]
    • view in new tab

    genes
    [gene|AC64DA463250A090A62E50901EFE653C8F963872|rsbV]-[gene|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|rsbW]-[gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]
    description
    [Pubmed|2170324]

    regulation

  • ''[protein|search|rsbV]:'' induced by stress ([protein|search|SigB]) [Pubmed|11544224]
    • view in new tab

    Biological materials

    Mutant

  • QB5344 (cat), available in the [SW|Stlke] lab
  • 1A675 ( ''sigB''::''cat''), [Pubmed|3027048], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A675&Search=1A675 BGSC]
  • 1A777 ( ''sigB''::''cat''), [Pubmed|1744042], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A777&Search=1A777 BGSC]
  • 1A780 ( ''sigB''::''spec''), [Pubmed|8253681], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A780&Search=1A780 BGSC]
  • BKE04730 ([gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTATGAGCCGATCGACTT, downstream forward: _UP4_GATCCCTCGATGGAGTTAAT
  • BKK04730 ([gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTATGAGCCGATCGACTT, downstream forward: _UP4_GATCCCTCGATGGAGTTAAT

    • Labs working on this gene/protein

  • [SW|Bill Haldenwang], San Antonio, USA
  • [SW|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]

    • References

    Reviews

  • 19575568,18035607,11407115,9767581,20658979,27518094,28271471

    • Control of SigB activity by protein-protein interactions

  • 11377871,12270815,15312768,8460143,8682769,8002610,11591687,7601843,17498739,15342582

    • Identification of the SigB regulon

  • 11544224,11532142,11717291,10482513,10220166,22174379

    • Other publications

  • 23407164,22511268,22210769,23524614,21979936,8655572,3123466,10369900,3112122,8468294,8458834,13129942,6784117,12867438,3100810,8253681,12486038,8764398,3027048,15342585,17575448,17586624,23934352,25652417,3016731,11902719,14651641,10503549,15205443,6405278,10383961,15805528,6790515,116131,39767581,19948797,22582280,24572018,24878601,27977677,28461450,11955433,28727759,29454936,30396900